The specific requirement for sodium chloride for the active uptake of L-glutamate by Halobacterium salinarium.
نویسنده
چکیده
1. Uptake of l-glutamate by Halobacterium salinarium is dependent on high concentrations of sodium chloride in the environment. When the sodium chloride is replaced by isomolar concentrations of potassium chloride, sodium acetate or potassium acetate, only negligible uptake occurs. 2. Most of the glutamate taken up can be shown to be in the cells in the free state and at a concentration of at least 50 times that in the medium. Sodium chloride is therefore required for an active transport of the glutamate into the cells. 3. The question whether sodium chloride is essential for the actual migration of glutamate across the cell envelope or for the mechanism supplying energy for this migration is discussed on the basis of experiments on endogenous respiration and with inhibitors.
منابع مشابه
Partial purification and properties of Halobacterium cutirubrum L-alanine dehydrogenase.
1. Halobacterium cutirubrum L-alanine dehydrogenase was purified approx. 100-fold. 2. It has a mol. wt. of 72 500, about one-third that of two well-studied alanine dehydrogenases from non-halophiles. 3. The activity of the enzyme increases with temperature up to 70 degrees C, but the protein itself is not thermostable. 4. In the reductive amination reaction, the enzyme is fully active in the pr...
متن کاملGel-filtration and Density-gradient-centrifugation Studies of the Molecular Weights of Halobacterium Cutirubrum
1. Conditions have been established for the estimation of molecular weights of proteins by analytical gel filtration and sucrose-density-gradient centrifugation in 2.5M-potassium chloride-l M-sodium chloride; Halobacterium cutirubrum polynucleotide phosphorylase, DNA-dependent RNA polymerase and RNA-dependent RNA polymerase have been studied by these methods. 2. The RNA-dependent polymerase has...
متن کاملSynthesis and Characterization of Cation Exchange PVA-g-PAA/PBI sulfone Membrane for the Electrolysis of Sodium Chloride
The reported work is on the preparation and characterization of polyvinyl alcohol -g- Polyacrylic acid (PVA-g-PAA)/ Polybenzimidazole sulfone (PBIs) cation exchange membrane used for the electro dialysis of sodium chloride solution. The polymer syrup has been prepared using the grafting method with the help of polyvinyl alcohol and polyacrylic acid in the presence of water solvent. The polymer ...
متن کاملHalophilic enzymes in cell-free extracts of Halobacterium salinarium.
Cells of the obligately halophilic bacterium Halobacterium salinarium have been shown to contain high concentrations of sodium and potassium chloride (Christian and Ingram, J. Gen. Microbiol. 20:27, 1959; Christian and Waltho, Biochim. Biophys. Acta 65:506, 1962). Enzymes extracted from this organism require up to 25% NaCl for optimal activity (Baxter and Gibbons, Can. J. Microbiol. 2:599, 1956...
متن کاملArginine deiminase from Halobacterium salinarium. Purification and properties.
Arginine deiminase from the extreme halophilic archaebacterium Halobacterium salinarium was purified to homogeneity in a four-step procedure with a 310-fold enrichment. The enzyme consists of two identical subunits of 55 kDa; its native molecular mass is 105 kDa. The pI of 4.7 indicates that acidic nature of the protein, which is evidenced by its amino acid composition, which shows an excess of...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- The Biochemical journal
دوره 99 2 شماره
صفحات -
تاریخ انتشار 1966